Histomonas meleagridis, the causative agent of histomoniasis, is a protozoan parasite classified in the Dientamoebidae (order Tritrichomonadida). The α-tubulin gene of 7 H. meleagridis isolates originating from either domestic chickens or turkeys from the United States was amplified by nested PCR and sequenced. A 91.4–99.8% nucleotide identity was shared among the 7 different sequences, and phylogenetic analysis disclosed that the 7 isolates were divided into at least 3 clades. These sequences had a 91–99% nucleotide identity and a 96–100% amino acid identity compared with 3 H. meleagridis α-tubulin sequences obtained from isolates originating from turkeys in Germany. Further α-tubulin gene analysis from species in the Dientamoebidae will be useful in elucidating the evolutionary relationship of these protozoans.
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1 April 2011
Partial Sequence of the Alpha-Tubulin Gene From Histomonas meleagridis Isolates From the United States
Richard W. Gerhold,
Lori A. Lollis,
Larry R. McDougald,
Robert B. Beckstead
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Journal of Parasitology
Vol. 97 • No. 2
April 2011
Vol. 97 • No. 2
April 2011