A 4×4 factorial experiment was conducted to determine the effects of salinity (0.2‰, 11‰, 21‰, and 31‰) and temperature (20°C, 24°C, 28°C, and 32°C) on survival, growth, and energy budget of juvenile Litopenaeus vannamei with the initial wet body weight of 0.274–0.283 g. The experiment lasted for 5 wk. The results showed that all shrimp survived at 11‰, 21‰, and 31‰, irrespective of temperature; at 0.2‰, survival decreased with increasing temperature from 20°C to 28°C, then plateaued at 32°C. At all levels of temperature with increases of salinity within the range tested, specific growth rate or food consumption gradually increased, reaching the maximum value and thereafter declined. At 0.2‰, specific growth rate, food consumption, or apparent digestibility coefficient exhibited an increasing trend from 22°C, reaching the maximum value at 28°C, then showed a decline up to 32°C with increasing temperature, whereas at salinities of 11‰, 21‰, and 31‰; specific growth rate, food consumption, or apparent digestibility coefficient exhibited an increasing trend. By contrast, feed efficiency generally decreased in response to increasing temperature within the salinity range tested.