Spectroscopic Analysis of Hemolymph from the American Lobster (Homarus americanus)

Jason C. Bolton; Scott Collins; Rosemary Smith; Brian Perkins; Rod Bushway; Robert Bayer; John Vetelino

doi:10.2983/035.028.0421

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Jason C. Bolton,^1,* Scott Collins,^2,3 Rosemary Smith,^4,3 Brian Perkins,¹ Rod Bushway,¹ Robert Bayer,⁵ John Vetelino^4,3

¹1Department of Food Science and Human Nutrition, University of Maine, Orono, ME 04473
²2Department of Chemistry, University of Maine, Orono, ME 04473
³4Laboratory of Surface Science and Technology, University of Maine, Orono, ME 04473
⁴3Department of Electrical and Computer Engineering, University of Maine, Orono, ME 04473
⁵5Department of Animal and Veterinary Science and Lobster Institute, University of Maine, Orono, ME 0

^*Corresponding author. E-mail: Jason_Bolton@umit.maine.edu

Abstract

Hemolymph from the American lobster (Homarus americanus) was analyzed by optical spectroscopy for the concentration of total hemolymph proteins and results were compared with a more traditional technique: refractometry. Absorbance peaks at 280 nm for total hemolymph protein concentration and 334 nm for hemocyanin concentration were studied, compared with equivalent refractometry readings, and correlated to the vitality of the lobster. These spectroscopic measurements constitute engineering data in the design a future noninvasive, field-deployable microinstrument to sort lobster according to their health and long-term viability.

Citation Download Citation

Jason C. Bolton, Scott Collins, Rosemary Smith, Brian Perkins, Rod Bushway, Robert Bayer, and John Vetelino "Spectroscopic Analysis of Hemolymph from the American Lobster (Homarus americanus)," Journal of Shellfish Research 28(4), 905-912, (1 December 2009). https://doi.org/10.2983/035.028.0421

Published: 1 December 2009

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