Biological and ecological research is seriously handicapped because of difficulties experienced in the reliable detection and quantification of bivalve larvae. This is a critical issue in the case of the Manila clam Ruditapes philippinarum (Adams & Reeve, 1850), a largely invasive and commercially relevant species, with important wild, cultured, and naturalized populations throughout the world. A SYBR Green real-time PCR assay, containing TPHI16S1F and TPHI16S2R primers (specific to R. philippinarum female mtDNA), was designed and tested to provide a rapid and high-throughput PCR-based method for larval quantification. Accurate estimations of larval numbers in spiked plankton samples point to the usefulness of this system. It can be used through a wide range of evaluated variable experimental conditions, such as the presence of closely related bivalve species, stationary-dependent plankton abundance, sampling volumes, and larval size.
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1 December 2011
Quantification of Manila Clam Ruditapes philippinarum (Adams & Reeve, 1850) Larvae Based on SYBR Green Real-Time Polymerase Chain Reaction
Javier Quinteiro,
L. Pérez-Diéguez,
A. Sánchez,
R. I. Perez-Martín,
C. G. Sotelo,
M. Rey-Méndez
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Journal of Shellfish Research
Vol. 30 • No. 3
December 2011
Vol. 30 • No. 3
December 2011
16S rRNA
larvae detection
larvae quantification
Manila clam
real-time PCR
Ruditapes philippinarum
SYBR green