Research programs in mosquito-borne diseases have necessitated the bloodfeeding of mosquitoes in captivity. Parafilm has been shown to be an effective membrane through which mosquitoes can access blood, and numerous feeding devices using parafilm membranes have been reported. However, these devices can be incompatible with experimental conditions, which may require small blood volumes, exact and constant blood temperatures, and inexpensive or disposable materials. We report herein on 2 methodologies that can be used to make parafilm blood bags with a support side and a feeding side made up of a thinner sheet of parafilm. Blood bags were easily and cheaply made to handle volumes as little as 150 µl or as large as 5 ml, to be disposable, and to be easily fastened to a heat source to provide constant and reproducible blood temperatures. Blood bags represent perhaps the most versatile and inexpensive approach to parafilm membrane feeding yet reported.
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Vol. 23 • No. 2