With the objective of providing knowledge about the natural habitat of mosquito larvae, we conducted a study on the culicid fauna and identified larval habitat types at 3 different locations in the Serra do Mar State Park, Brazil, over the 12 months of 1991. We collected 1,425 larvae belonging to 23 species, of which the most frequent were Culex iridescens, Culex spp., Limatus durhami, and Trichoprosopon pallidiventer. The mosquito larval habitats presenting the greatest densities of specimens were bamboo, bromeliads, rubber boots, and streams. We observed that the culicids used a variety of containers as larval habitats and bred under a diversity of ecological conditions. Most of the habitats were natural, formed by bamboo, bromeliads, streams, and depressions in the ground and in rocks, along with artificial habitats consisting of rubber boots and abandoned metal cans. Seven species occurred preferentially in the internodes of closed bamboo stems, 6 in internodes of open stems, and 4 in bromeliads.