We evaluated Bactimos PT (Bacillus thuringiensis subsp. israelensis [Bti]) against larval populations of Glyptotendipes paripes at 30 kg/ha in man-made lakes on Hilton Head Island, SC. Three treatments were “whole-pond” treatments, while the 4th consisted of treating a “band” along the edge of a pond where significant larval populations had been observed. Larval populations were reduced by an average of 95% at day 7, 70% at day 14, and 50% at day 21 posttreatment in the whole-pond treatments. Initial larval suppression with the band treatment was similar to the whole-pond treatments, indicating that suppression activities can be targeted to specific areas of a larval habitat.