We compared the differential display of Aedes albopictus cells, both resistant and susceptible to Bacillus thuringiensis israelensis (Bti), using differentially displayed reverse transcription polymerase chain reaction. We found 1 band about 200 base pairs long. After cloning and sequencing, the differentially expressed gene was similar to some partial messenger ribonucleic acid of Ae. aegypti, Culex quinquefasciatus, and Anopheles gambiae, rather than Ae. albopictus. This will be of some value for clarifying the mechanism of mosquito resistance to Bti products.
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1 December 2012
A Differentially Displayed mRNA Related to Resistance to Bacillus thuringiensis israelensis of Aedes albopictus Selected in Vitro-Activated CYT1AA6
Lingling Zhang,
Qi Zhang,
Enjiong Huang,
Mingwei Li,
Tianpei Huang,
Lei Xu,
Chanbiao Wu,
Xiong Guan,
Ivan Gelbič
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Aedes albopictus
Bacillus thuringiensis israelensis
C6/36 cells
differentially displayed reverse transcription polymerase chain reaction
resistance
