Using novel DNA sequence data, we designed a restriction enzyme assay that distinguishes Aedes atlanticus and Ae. tormentor, based on size polymorphisms. The restriction endonuclease Hpy188I digests polymerase chain reaction–amplified 2nd internal transcribed spacer products once for Ae. atlanticus and twice for Ae. tormentor, thus providing a useful method for identifying adult female collections that are generally considered morphologically indistinguishable.
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