Here we describe an undergraduate microbiology laboratory exercise that examines differences between an anthropogenic and a natural soil microbial community. These two microbiological communities were analyzed using community level physiological profiling, a quantitative method for describing the microbial composition of the bacterial community using carbon source utilization. Specifically, Biolog EcoPlate Microplates were used to determine quantitatively the use of 31 different carbon sources for oxidative respiration by each community. Carbon source utilization was compared between communities and within communities using univariate statistical techniques. The utility of this exercise in teaching how statistical analysis is informative in the biological sciences is discussed, as well as strategies to include a range of statistical analysis from descriptive and univariate techniques to multivariate approaches.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 40 • No. 2