Life history of Eufriesea smaragdina (Perty, 1833) (Euglossina) was studied with trap nests. Over 4 yr bamboo canes and wooden blocks were placed in trees in secondary Atlantic forest on Santa Catarina Island and restinga forest on the adjacent mainland in Santa Catarina, southern Brazil. Forty-one brood cells were obtained in 14 nests and 23 Ef. smaragdina imagines emerged in November and December suggesting a univoltine life cycle for these bees. One cleptoparasitic orchid bee, Exaerete dentata, and a meloid beetle, Tetraonyx sp., were also reared from the nests. Their emergence fitted into the schedule of Ef. smaragdina suggesting a close adaptation to this host.