The aquatic macroinvertebrates of Sand Creek, a first order Front Range Colorado stream, were surveyed from March–September 2012 with an additional winter adult collecting trip on 2 March 2013 using both qualitative and quantitative techniques, emphasizing EPT (Ephemeroptera/Plecoptera/Trichoptera) taxa. One hundred and thirty macroinvertebrate taxa were identified from both qualitative samples and quantitative benthic samples. Of these, 53 mayfly/stonefly/caddisfly species were collected from Sand Creek, a remarkable number for such a small Front Range stream. The macroinvertebrate community of Sand Creek is dominated by collector-gatherers (50–76%), collector-filterers (9–33%) and shredders (3–24%), with the other feeding guilds comprising low overall percentages of the community. The collector-gatherer taxa were primarily mayfly taxa, chironomid midges, stratiomyid flies, the elmid genus Optioservus, and oligochaete worms; collector-filterers were primarily net spinning hydropsychid caddisflies; and shredders were primarily nemourid stoneflies. Sand Creek should be protected from cattle grazing which causes degradation of the riparian zone and stream bank integrity.