We provide information on the nesting behavior, natural enemies, seasonality, and host plants of Anthophora (Dasymegilla) waltoni Cockerell at mid-elevations in southern China (Yunnan Province). Bees nested singly inside loose mortar joints of building walls. Nests were shallow and consisted of an unlined, unbranched tunnel ending in a single cell. Surveys conducted every two or three days from February to April, 2013, showed that the studied population is univoltine, protandrous, and active in early spring. Females were more commonly observed than males, and no bees were observed flying by the end of April. Both sexes of A. waltoni were often collected on flowers of Vicia cracca Linnaeus (Fabaceae).