Eastern mistletoe [Phoradendron leucarpum (Raf.) Reveal & M.C. Johnston] was observed on 1104 host trees from 10 species and 8 families in Jessamine County, east-central Kentucky within the Inner Bluegrass and Hills of the Bluegrass Ecoregions. Juglans nigra was the major host tree (609 trees) followed by Prunus serotina (259 trees), Ulmus americana (116 trees), Robinia pseudoacacia (43 trees) and Celtis occidentalis (39 trees). The top three host species accounted for 89.1% of the infested trees. Eastern mistletoe exhibits an aggregated or clumped spatial distribution pattern among host trees characteristic of its life history and avian fruit and seed dispersal.