Nineteen locations were sampled in the Obion Creek drainage from July 2007 until May 2008 to determine species composition of fishes in the channelized drainage in western Kentucky and evaluate anthropogenic disturbances with the Kentucky Index of Biotic Integrity. The Kentucky Index of Biotic Integrity (KIBI) was used to evaluate 17 of the 19 locations. Survey results of the Obion Creek drainage lists18 families represented by 65 species. The Obion Creek drainage is in the Jackson Purchase area of western Kentucky; an area where a majority of the land is in heavy agricultural use.

Five species collected were considered rare by the Kentucky State Nature Preserves Commission: blacktail shiner (Cyprinella venusta), taillight shiner (Notropis maculatus), lake chubsucker (Erimyzon succeta), chain pickerel (Esox niger), and central mudminnow (Umbra limi). Three species collected were considered exotic and highly invasive: grass carp (Ctenopharyngodon idella), silver carp (Hypophthalmichthys molitrix), and the common carp (Cyprinus carpio). Species richness has increased over previous collections in a 40 year period. Kentucky Index of Biotic Integrity scores ranged from fair to poor in the Obion Creek drainage.