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Isospora buteonis Henry 1932 was considered the probable cause of death in an American kestrel (Falco sparveius) and a possible cause of death in a golden eagle (Aquila chrysaëtos) in the state of Washington, U.S.A. I. buteonis has been reported only from the western part of North America. It resembles I. bigemina in size, in location and sporulation of oocysts beneath the intestinal epithelium, and in fragility of the oocyst wall.
The golden shiner, Notemigonus crysoleueas, developed antibody titer of 1,280 in 59 days from multiple, intraperitoneal injections of a formalin-killed bacterin of Acromonas liquefaciens. After four immunizing injections there was a significant increase in the time of survival of vaccinated fish over nonvaccinated fish challenged with viable A. liquefaciens. Analysis of golden shiner sera by paper electrophoresis produced a pattern of six protein components. Antibody activity was associated with a component which had a slow anodic electrophoretic mobility and solubility of gamma globulin. During the course of vaccination, increments in the serum protein component and in antibody titer coincided. Also, the amount of the protein component of vaccinated fish was decreased by specific adsorption of antibody by homologous antigen. Thus, a fish immunoglobulin was identified which had certain physical and chemical properties correspondng to classical definitions of gamma globulin.
Pox lesions were described in three new species of wild birds: Swiainson's thrush (Hylocichla ustulata), gray-cheeked thrush (Hylocichla minima) and brown creeper (Certhia familiaris). These birds were cellected during bird banding activities on Long Point, Ontario.
Hemagglutination-inhibition (HI) tests of serum samples from 51 bighorn sheep (Ovis canadensis) and 4 bighorn-domestic hybrid sheep from Wyoming and Montana revealed bovine myxovirus parainfluenza-3 antibody titers of 1:20 or greater in 6 animals. The serums of two bighorns with pneumonia were negative for neutralizing antibodies against IBR virus and the serums of 9 animals failed to show significant titers against 2 strains of human Asian influenza virus. Tissue cultures of nasal swabs and lung tissue were negative for cytopathogenic effects and hemadsorption.