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1 July 1985 DETECTION OF VIBRIO ANGUILLARUM ANTIGEN BY THE DOT BLOT ASSAY
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Abstract

The dot blot assay, modified and adapted for detection of antigens from Vibrio anguillarum in fish tissues, was specific for V. anguillarum and did not react with antigens of V. ordalii, Pseudomonas sp., or Yersinia ruckeri. The blot assay enabled detection of as little as 2.3 ng of a mixture of protein antigens obtained from cell-free extracts of V. anguillarum; it was about 100 times more sensitive than either the indirect fluorescent antibody technique or bacterial isolation for detecting V. anguillarum in fish tissues.

Cipriano, Pyle, Starliper, and Pyle: DETECTION OF VIBRIO ANGUILLARUM ANTIGEN BY THE DOT BLOT ASSAY
R. C. Cipriano, J. B. Pyle, C. E. Starliper, and S. W. Pyle "DETECTION OF VIBRIO ANGUILLARUM ANTIGEN BY THE DOT BLOT ASSAY," Journal of Wildlife Diseases 21(3), 211-218, (1 July 1985). https://doi.org/10.7589/0090-3558-21.3.211
Received: 26 October 1984; Published: 1 July 1985
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