The prevalence of Sarcocystis (Protozoa: Sarcocystidae) in white-tailed deer (Odocoileus virginianus) from northeastern Minnesota was determined by histologic examination of tongue samples. Seventy-nine of 100 deer were infected; infection was higher in yearlings and adults than in fawns. Sporocysts of Sarcocystis were found in 3% of 72 wolf (Canis lupus) scats. Three of four captive wolves fed muscle from a white-tailed deer naturally infected with Sarcocystis shed sporocysts 12–14 days later.