Blood from endangered San Joaquin kit foxes (Vulpes macrotis mutica) inhabiting the Elk Hills Naval Petroleum Reserve, Kern County, and the Elkhorn Plain, San Luis Obispo County, California, was collected in 1981, 1982 and 1984 and sera were tested for antibodies against 10 selected pathogens. Proportions of kit fox sera containing antibodies against pathogens were: canine parvovirus, 100% in 1981–1982 and 67% in 1984; infectious canine hepatitis virus, 6% in 1981–1982 and 21% in 1984; canine distemper virus, none in 1981–1982 and 14% in 1984; Francisella tularensis, 8% in 1981–1982 and 31% in 1984; Brucella abortus, 8% in 1981–1982 and 3% in 1984; Brucella canis, 14% in 1981–1982 and none in 1984; Toxoplasma gondii, 6% in 1981–1982; Coccidioides immitis, 3% in 1981–1982; and Yersinia pestis and Leptospira interrogans serotypes canicola, grippotyphosa, hardjo, icterohaemorrhagiae, and pomona, none in 1981–1982. Although antibodies against selected pathogens were present, no clinical indications of disease were observed in these fox populations.