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1 April 1990 SEROLOGICAL INVESTIGATION OF THE FISH PATHOGEN EDWARDSIELLA ICTALURI, CAUSE OF ENTERIC SEPTICEMIA OF CATFISH
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Abstract

The serological relationships among 32 isolates of Edwardsiella ictaluri obtained from fish were studied. The strains were extremely homogeneous in protein and lipopolysaccharide preparations as observed by sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. Only minor variations were observed in the structural O-side chain subunits in three isolates; however, such variation did not preclude antigenic recognition by two E. ictaluri antisera in either microagglutination or Western blot immunoassays. The antigenic homogeneity of E. ictaluri was further demonstrated by microagglutination assays with both formalin-killed and heat inactivated cellular antigens. The minimal degree of antigenic variability observed suggested that most isolates of E. ictaluri compose a single antigenic serotype.

Bertolini, Cipriano, Pyle, and McLaughlin: SEROLOGICAL INVESTIGATION OF THE FISH PATHOGEN EDWARDSIELLA ICTALURI, CAUSE OF ENTERIC SEPTICEMIA OF CATFISH
James M. Bertolini, Rocco C. Cipriano, S. W. Pyle, and John J. A. McLaughlin "SEROLOGICAL INVESTIGATION OF THE FISH PATHOGEN EDWARDSIELLA ICTALURI, CAUSE OF ENTERIC SEPTICEMIA OF CATFISH," Journal of Wildlife Diseases 26(2), 246-252, (1 April 1990). https://doi.org/10.7589/0090-3558-26.2.246
Received: 26 May 1988; Published: 1 April 1990
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