A combination of the dissociative anesthetic ketamine hydrochloride (KH) and the sedative xylazine hydrochloride (XH) was used to immobilize 31 wild Iberian lynx (Felis pardina) 45 times at Doñana National Park, Spain. A mean (±SE) dose of 4.6 (±0.2) mg/kg KH and 4.0 (±0.2) mg/kg XH resulted in mean (±SE) induction time of 5.6 (±0.3) min and mean (±SE) first reaction time of 59.3 (±6.5) min. Convulsions occurred four times (9%), but with no noteworthy consequences.