During the slaughtering season of February and March 1991, 559 capybaras (Hydrochoerus hydrochaeris) were tested for Trypanosoma evansi in two areas in Venezuela: El Frio Ranch and El Cedral Ranch. Blood and serum samples were evaluated for T. evansi. Forty-eight (9%) of 559 capybaras had T. evansi using the microcentrifugation technique. Further, 279 (50%) of the 559 capybaras had antibodies against T. evansi immunofluorescence test in both ranches. Thus, capybaras may be important in the epizootiology of T. evansi in this enzootic area.