The 5′ untranslated region (5′ UTR) of cytopathogenic pestiviruses isolated from two seronegative roe deer (Capreolus capreolus) in northern Germany was partially sequenced and compared with those of 28 other pestiviruses. Due to the occurrence within a narrow location and the complete identity of the sequenced fragments from both roe deer isolates (SH9 and SH11) they seem to belong to the same bovine virus diarrhea virus (BVDV) strain called SH9/11. This strain is highly homologous (up to 93% identity) to “classical” BVDV strains. However, SH9/11 has characteristic variations in its 5′ UTR distinct from all other pestiviruses analyzed in this study. Strain SH9/11 is more similar to BVDV group I than to group II, although it is clearly separated from all other cattle isolates tested. In monoclonal antibody (mAb)-typing studies, isolate SH9 reacted with one pestivirus-specific mAb (C16), with two BVDV specific mAbs (N2B12 and D5), and with one mAb (f48) raised against the E2 protein of classical swine fever virus out of a panel of 13 mAbs. The separate position of strain SH9/11 again was demonstrated by the unique reaction pattern of isolate SH9 when compared with other mAb f48-positive BVDV and BDV strains. All these results indicate that distinct BVDV strains might exist among freeranging roe deer in Germany.
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Vol. 34 • No. 1