Translator Disclaimer
1 April 2002 ANALYSIS OF MITOGEN-STIMULATED LYMPHOCYTE SUBSET PROLIFERATION AND NITRIC OXIDE PRODUCTION BY PERIPHERAL BLOOD MONONUCLEAR CELLS OF CAPTIVE ELK (CERVUS ELAPHUS)
Author Affiliations +
Abstract

Elk (Cervus elaphus) are reservoirs for Brucella abortus, Mycobacterium bovis, and Mycobacterium avium subsp. paratuberculosis, each a serious pathogen of domestic livestock. An understanding of the basic immune responsiveness of elk would aid efforts to develop methods to diagnose and prevent these diseases of elk. Peripheral blood mononuclear cells (PBMC) isolated from captive elk were examined for phenotype, lymphocyte subset proliferative capacity, and ability to produce nitric oxide (NO) upon pokeweed mitogen (PWM) stimulation. Although γδ TCR cells represented a high percentage of the peripheral blood lymphocyte pool, these cells responded poorly to PWM stimulation. B cells (i.e., sIgM cells), conversely, were responsive to PWM stimulation. Addition of PWM to PBMC cultures also resulted in a significant production of nitrite, the stable oxidation product of NO. Similar to other ruminant species, the majority of elk peripheral blood sIgM cells co-expressed MHC class II and B-B4, a B cell lineage marker that varies in expression during B cell development. Findings from the present study provide basic information on several parameters of cellular immunity of elk.

Waters, Sacco, Fach, Palmer, Olsen, and Kreeger: ANALYSIS OF MITOGEN-STIMULATED LYMPHOCYTE SUBSET PROLIFERATION AND NITRIC OXIDE PRODUCTION BY PERIPHERAL BLOOD MONONUCLEAR CELLS OF CAPTIVE ELK (CERVUS ELAPHUS)
W.R. Waters, R. E. Sacco, S. J. Fach, M. V. Palmer, S. C. Olsen, and T. J. Kreeger "ANALYSIS OF MITOGEN-STIMULATED LYMPHOCYTE SUBSET PROLIFERATION AND NITRIC OXIDE PRODUCTION BY PERIPHERAL BLOOD MONONUCLEAR CELLS OF CAPTIVE ELK (CERVUS ELAPHUS)," Journal of Wildlife Diseases 38(2), 344-351, (1 April 2002). https://doi.org/10.7589/0090-3558-38.2.344
Received: 9 May 2001; Published: 1 April 2002
JOURNAL ARTICLE
8 PAGES


SHARE
ARTICLE IMPACT
Back to Top