Little is known about the prevalence or importance of Toxoplasma gondii infections in raptors. Sera from Eurasian Buzzards (Buteo buteo, n=14), Tawny Owls (Strix aluco, n=12), Barn Owls (Tyto alba, n=18), Eurasian Sparrowhawk (Accipiter nisus, n=1), and Common Kestrels (Falco tinnunculus, n=8) were examined for agglutinating antibodies using the modified agglutination test at 1:25 dilution. Antibodies were not detected in Common Kestrels and the Eurasian Sparrowhawk but were detected in 11 Eurasian Buzzards (79%), six Tawny Owls (50%), and two Barn Owls (11%). Toxoplasma gondii, genotype II, was isolated from the brain of an adult Tawny Owl.