Intensity of hematozoan infection is infrequently quantified because accurate calculations require visual counts of parasites relative to a large number of erythrocytes. Manual quantification of erythrocytes can be circumvented by using ImageJ software (developed by the National Institutes of Health) to count erythrocyte nuclei from digital images. Here we use the ratio of microscope erythrocyte counts to digital image erythrocyte counts (field:image ratio) to extrapolate erythrocyte counts from smaller digital images to the microscope's larger field of view. Field:image ratios were consistently calculated from 10 slides (resampling P=0.049) and used to rapidly estimate intensity of infection within 50,000 or more erythrocytes. Intensity of hematozoan infection calculated from manual quantification of 2,000 erythrocytes was significantly lower (0.46 times) than intensity calculated from digital quantification of 50,000 erythrocytes (bootstrap P=0.02). We contend that digital quantification of hematozoan infection offers a rapid and precise method to quantify infections of low to moderate intensity.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.