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1 April 2011 Detection of Low Pathogenic Avian Influenza Viruses in Wild Ducks from Canada: Comparison of Two Sampling Methods
E. Jane Parmley, Catherine Soos, André Breault, Madeleine Fortin, Emily Jenkins, Fred Kibenge, Robin King, Keith McAloney, John Pasick, Sydney Paul Pryor, John Robinson, Jean Rodrigue, Frederick A. Leighton
Author Affiliations +
Abstract

Surveillance for avian influenza viruses in wild birds was initiated in Canada in 2005. In 2006, in order to maximize detection of highly pathogenic avian influenza viruses, the sampling protocol used in Canada's Inter-agency Wild Bird Influenza Survey was changed. Instead of collecting a single cloacal swab, as previously done in 2005, cloacal and oropharyngeal swabs were combined in a single vial at collection. In order to compare the two sampling methods, duplicate samples were collected from 798 wild dabbling ducks (tribe Anatini) in Canada between 24 July and 7 September 2006. Low pathogenic avian influenza viruses were detected significantly more often (P<0.0001) in combined oropharyngeal and cloacal samples (261/798, 33%) than in cloacal swabs alone (205/798, 26%). Compared to traditional single cloacal samples, combined samples improved virus detection at minimal additional cost.

E. Jane Parmley, Catherine Soos, André Breault, Madeleine Fortin, Emily Jenkins, Fred Kibenge, Robin King, Keith McAloney, John Pasick, Sydney Paul Pryor, John Robinson, Jean Rodrigue, and Frederick A. Leighton "Detection of Low Pathogenic Avian Influenza Viruses in Wild Ducks from Canada: Comparison of Two Sampling Methods," Journal of Wildlife Diseases 47(2), 466-470, (1 April 2011). https://doi.org/10.7589/0090-3558-47.2.466
Received: 20 May 2010; Accepted: 1 November 2010; Published: 1 April 2011
KEYWORDS
avian influenza
Canada
cloacal
detection
oropharyngeal
sampling
swab
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