Translator Disclaimer
1 July 2011 Domestic Dog Origin of Canine Distemper Virus in Free-ranging Wolves in Portugal as Revealed by Hemagglutinin Gene Characterization
Alexandra Müller, Eliane Silva, Nuno Santos, Gertrude Thompson
Author Affiliations +
Abstract

Serologic evidence for canine distemper virus (CDV) has been described in grey wolves but, to our knowledge, virus strains circulating in wolves have not been characterized genetically. The emergence of CDV in several non-dog hosts has been associated with amino acid substitutions at sites 530 and 549 of the hemagglutinin (H) protein. We sequenced the H gene of wild-type canine distemper virus obtained from two free-ranging Iberian wolves (Canis lupus signatus) and from one domestic dog (Canis familiaris). More differences were found between the two wolf sequences than between one of the wolves (wolf 75) and the dog. The latter two had a very high nucleotide similarity resulting in identical H gene amino acid sequences. Possible explanations include geographic and especially temporal proximity of the CDV obtained from wolf 75 and the domestic dog, taken in 2007–2008, as opposed to that from wolf 3 taken more distantly in 1998. Analysis of the deduced amino acids of the viral hemagglutinin revealed a glycine (G) and a tyrosine (Y) at amino acid positions 530 and 549, respectively, of the partial signaling lymphocytic activation molecule (SLAM)-receptor binding region which is typically found in viral strains obtained from domestic dogs. This suggests that the CDV found in these wolves resulted from transmission events from local domestic dogs rather than from wildlife species.

Alexandra Müller, Eliane Silva, Nuno Santos, and Gertrude Thompson "Domestic Dog Origin of Canine Distemper Virus in Free-ranging Wolves in Portugal as Revealed by Hemagglutinin Gene Characterization," Journal of Wildlife Diseases 47(3), 725-729, (1 July 2011). https://doi.org/10.7589/0090-3558-47.3.725
Received: 10 September 2010; Accepted: 1 February 2011; Published: 1 July 2011
JOURNAL ARTICLE
5 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top