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1 October 2011 Prevalence and Molecular Characterization of Mycoplasma ovis in Selected Free-Ranging Brazilian Deer Populations
Ana Laura Grazziotin, José Maurício Barbanti Duarte, Matias Pablo Juan Szabó, Andrea Pires Santos, Ana Marcia Sa Guimarães, Ahmed Mohamed, Rafael Felipe da Costa Vieira, Ivan Roque de Barros Filho, Alexander Welker Biondo, Joanne Belle Messick
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Abstract

Mycoplasma ovis is a hemoplasma that may cause anemia and mortality in small ruminants. Our aim was to determine whether M. ovis infects populations of free-ranging deer in Brazil. Buffy coat samples from 64 Blastocerus dichotomus from Porto Primavera, 18 Ozotocerus bezoarticus from Pantanal, and 21 O. bezoarticus from Emas National Park were tested. Using a M. ovis PCR protocol to amplify extracted DNA, 46/64 (72%) of deer from Porto Primavera, 10/18 (56%) from Pantanal, and 4/21 (19%) from Emas National Park were positive, giving an overall positive rate of 58% for hemoplasma in these wild deer. Sequencing and phylogenetic analysis of the 16S rRNA gene revealed 3 genetically distinct hemoplasmas including M. ovis, ‘Candidatus Mycoplasma erythrocervae’, and a hemoplasma most closely related to M. ovis. Phylogenetic analysis of the 23S rRNA gene from selected sequences confirmed these relationships.

Ana Laura Grazziotin, José Maurício Barbanti Duarte, Matias Pablo Juan Szabó, Andrea Pires Santos, Ana Marcia Sa Guimarães, Ahmed Mohamed, Rafael Felipe da Costa Vieira, Ivan Roque de Barros Filho, Alexander Welker Biondo, and Joanne Belle Messick "Prevalence and Molecular Characterization of Mycoplasma ovis in Selected Free-Ranging Brazilian Deer Populations," Journal of Wildlife Diseases 47(4), 1005-1011, (1 October 2011). https://doi.org/10.7589/0090-3558-47.4.1005
Received: 11 January 2011; Accepted: 1 June 2011; Published: 1 October 2011
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