A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis–infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×103 cells of M. bovis culture/g and Bacille Calmette–Guérin standards with 1.58×101 genome copies/well were positive by real-time PCR but all field samples were negative.
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1 January 2014
Utility of a Fecal Real-time PCR Protocol for Detection of Mycobacterium bovis Infection in African Buffalo (Syncerus caffer)
Annette Roug,
Claire Geoghegan,
Elizabeth Wellington,
Woutrina A. Miller,
Emma Travis,
David Porter,
David Cooper,
Deana L. Clifford,
Jonna A. K. Mazet,
Sven Parsons
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Journal of Wildlife Diseases
Vol. 50 • No. 1
January 2014
Vol. 50 • No. 1
January 2014