Northern Bobwhites (Colinus virginianus) have been in decline throughout the southeastern US. Prevalence of Trichomonas gallinae in wild bobwhites is unknown, although T. gallinae caused morbidity and mortality in experimentally infected bobwhites. Many species of Columbidae (pigeons and doves) in Texas are hosts to T. gallinae. Bobwhites potentially may become exposed to this protozoan through supplemental feed or water sources contaminated by columbids infected with T. gallinae. All of 506 bobwhites collected in Oklahoma and Texas, 2011–13, were PCR negative for T. gallinae. These data suggest T. gallinae is not contributing to the population decline of bobwhites in this region.