Direct Detection of Escherichia coli Virulence Genes by Real-Time PCR in Fecal Samples from Bats in Brazil

Adriana Cabal; Maria J. Pereira; Ludmilla M. S. Aguiar; Lucas Domínguez; Carlos Fonseca; Julio Álvarez; Jan F. Drexler; Christian Gortázar

doi:10.7589/2015-01-024

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Adriana Cabal,^1,2 Maria J. Pereira,^3,4,5 Ludmilla M. S. Aguiar,⁶ Lucas Domínguez,¹ Carlos Fonseca,⁵ Julio Álvarez,⁷ Jan F. Drexler,⁸ Christian Gortázar^2,*

¹1 VISAVET Health Surveillance Centre, Complutense University of Madrid, Avenida Puerta de Hierro s/n
²2 SaBio IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n 13071 Ciudad Real, Spain
³3 PPGBAN, Department of Zoology, Institute of Biosciences, Federal University of Rio Grande do Sul,
⁴4 PPGEC, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, 79090-900, Braz
⁵5 Department of Biology and Center for Environmental and Marine Studies, University of Aveiro, Campu
⁶6 Department of Zoology and Institute of Biological Sciences, University of Brasília, Campus Darcy R
⁷7 Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnes
⁸8 Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25 Bonn 53127, German

^*Corresponding author (email: Christian.Gortazar@uclm.es)

Abstract

Guano samples from 412 Brazilian bats were screened with real-time PCR for the virulence genes (eae, est, elt, stx1, stx2, ehxA, invA, bfpA, aggR) representing five intestinal pathotypes of Escherichia coli. From 82 pooled samples, 22% contained Escherichia coli DNA, and eae, est, bfpA, aggR were detected.

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Adriana Cabal, Maria J. Pereira, Ludmilla M. S. Aguiar, Lucas Domínguez, Carlos Fonseca, Julio Álvarez, Jan F. Drexler, and Christian Gortázar "Direct Detection of Escherichia coli Virulence Genes by Real-Time PCR in Fecal Samples from Bats in Brazil," Journal of Wildlife Diseases 51(4), 942-945, (1 October 2015). https://doi.org/10.7589/2015-01-024

Received: 30 January 2015; Accepted: 1 April 2015; Published: 1 October 2015

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