Translator Disclaimer
1 July 2017 Detection of Brucellosis in Sika Deer ( Cervus nippon) through Loop-mediated Isothermal Amplification (LAMP)
Author Affiliations +
Abstract

Brucellosis (Brucella bovis) in sika deer (Cervus nippon) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. It also poses a threat to humans because it is a zoonotic disease. Use of the loop-mediated isothermal amplification (LAMP) assay has been poorly described in the diagnosis of brucellosis in deer. We developed a LAMP assay targeting the omp25 gene sequence to detect brucellosis in sika deer. The reaction can be completed in 60 min at 63 C and, with a detection limit of 17 pg, it was more sensitive than conventional PCR, with its detection limit of 1.7 ng. No cross-reactivity was observed with four bacteria: Escherichia coli, Salmonella enterica subsp. enterica, Clostridium pasteurianum, and Pseudomonas aeruginosa. We used 263 samples of blood to evaluate the reaction. The percentage of agreement between LAMP and PCR reached 91%; relative specificity reached 87%, and relative sensitivity reached 100%. The results indicate LAMP can be a simple and rapid diagnostic tool for detecting brucellosis in sika deer, particularly in the field, where it is essential to control brucellosis in deer with a rapid and accurate diagnosis for removal of positive animals.

© Wildlife Disease Association 2017
Qianhong Liu, Jie Wei, Qingsong Sun, Ben Wang, Yuting Wang, Ying Hu, and Wenrong Wu "Detection of Brucellosis in Sika Deer ( Cervus nippon) through Loop-mediated Isothermal Amplification (LAMP)," Journal of Wildlife Diseases 53(3), 612-615, (1 July 2017). https://doi.org/10.7589/2016-05-105
Received: 12 May 2016; Accepted: 1 December 2016; Published: 1 July 2017
JOURNAL ARTICLE
4 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top