The transmission of multiple serotypes of epizootic hemorrhagic disease virus (EHDV) between farmed and free-ranging wildlife is of interest to livestock industries and natural resource agencies. We compared the seroprevalence of EHDV-1, -2, and -6 in wild and farmed white-tailed deer (Odocoileus virginianus) herds in Florida, US. We compared serological prevalence, circulating serotypes, antibody titers, and viremia with the use of 171 whole-blood samples from 150 unvaccinated white-tailed deer from farm pens, a farm preserve, and wild deer on adjacent public lands between March 2016 and May 2017. Despite aggressive chemical vector control on the farm, we found higher seroprevalence and titers against the predominant EHDV serotype in farmed deer (in pens and the preserve) than in wild deer. The higher exposure to EHDV of farmed vs. wild deer may have been because of the higher densities of farmed vs. wild deer, the presence of exotic amplifying hosts such as elk (Cervus canadensis) in the preserve, or genetic factors that predisposed farmed deer to disease.