The kidney, liver, intestine, brain, and muscle of live infected channel catfish were assayed for channel catfish virus in channel catfish gonad cell cultures. Sampling was done at 24-hour intervals for 120 hours. The virus was first detected in the kidneys of channel catfish 24 hours after inoculation. Channel catfish virus was also isolated at sequential time intervals from the intestine, liver, brain, and muscle. Virus was detected in the kidney, liver, and intestine but not the brain of the fish that died 70 hours after infection.