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1 March 2001 HUMORAL IMMUNE RESPONSES OF BLACK-FOOTED PENGUINS (SPHENISCUS DEMERSUS) AFTER DNA-MEDIATED IMMUNIZATION WITH A BETA-GALACTOSIDASE REPORTER GENE
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Abstract

Humoral immune responses of black-footed penguins (Spheniscus demersus) to DNA-mediated immunization with a β-galactosidase reporter gene expression plasmid were evaluated. Six male and 6 female adult penguins received either test plasmid, pCMV-β, containing the β-galactosidase gene or control plasmid, pCI, lacking a gene for expression. Three birds from each group were used previously in a diluent control group and given one injection of sterile saline. All samples were screened for anti-β-galactosidase antibodies by indirect enzyme-linked immunosorbent assay with anti-chicken immunoglobulin G as secondary antibody. Antibodies to β-galactosidase were detected in the sera of pCMV-β–inoculated penguins, with a peak response on day 21. Antibody titers of the test plasmid group versus both control groups on days 21, 28, and 42 differed significantly. These results demonstrate that black-footed penguins can be safely transfected with the gene encoding β-galactosidase and will mount a humoral response against the in vivo–expressed protein. Knowledge from this initial study can be applied to the development of DNA-mediated vaccines against specific infectious diseases of penguins.

Johanna Sherrill, Tracey Schock, J. Lawrence Dunn, David J. St Aubin, Vicki V. Burnley, and Steven E. Poet "HUMORAL IMMUNE RESPONSES OF BLACK-FOOTED PENGUINS (SPHENISCUS DEMERSUS) AFTER DNA-MEDIATED IMMUNIZATION WITH A BETA-GALACTOSIDASE REPORTER GENE," Journal of Zoo and Wildlife Medicine 32(1), 17-24, (1 March 2001). https://doi.org/10.1638/1042-7260(2001)032[0017:HIROBF]2.0.CO;2
Published: 1 March 2001
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