Using a crossover design, the effects of the addition of ketamine to a previously determined optimal hand-injected immobilization dosage of carfentanil/xylazine were evaluated in 11 adult white-tailed deer (Odocoileus virginianus). Two i.m. ketamine dosages were evaluated: 0.15 mg/kg (low ketamine) and 0.30 mg/kg (high ketamine). Each deer was immobilized twice 2 wk apart. Inductions were video recorded and reviewed by observers, who had been blinded to drugs and dosages, who rated qualitative aspects. There were significant (P < 0.05) dosage-dependent decreases in heart rate, SaO₂, and arterial pH, and a significant dosage-dependent increase in PaCO₂. Induction times with both dosages were more rapid (mean 2.3 ± 0.9 min for low ketamine and 2.3 ± 0.6 min for high ketamine) than those reported for the same carfentanil/xylazine dosage used without ketamine. Mean quality ratings, though improved compared to those reported for carfentanil/xylazine alone, were considered “undesirable” for both dosages. Hyperthermia (temperature >41°C) was noted in 13 of 22 immobilizations. Arterial pH and PaO₂ increased significantly from 10 to 20 min postrecumbency, but acidemia (pH <7.3) was present throughout immobilization periods for all deer. There were ketamine dosage-dependent increases in respiratory components of this acidemia compared with that associated with carfentanil/xylazine alone. Possible hypoxemia was present at both sampling times for both groups, while hypercapnea (PaCO₂ >60 mm Hg) was present for the high-ketamine group only. Reversal times for naltrexone and yohimbine were rapid (mean 2.9 ± 0.7 min for low ketamine and 3.3 ± 0.8 min for high ketamine), with no evidence of renarcotization. Although the addition of ketamine to carfentanil/xylazine caused faster inductions and improved induction qualities, it also produced an increased incidence of hyperthermia, acidemia, hypoxemia, and hypercapnea. Supplemental oxygen and close monitoring of body temperature is recommended when using this immobilization regimen.