Intraocular pressure was measured using rebound tonometry in American flamingos (Phoenicopterus ruber), with the head in an upright standing position and when lowered in a feeding position, to establish a reference range. Mean ± standard deviation (SD) (range) intraocular pressure for flamingos with the head in an upright position was right eye (OD) = 10.9 ± 1.8 mm Hg (7–15 mm Hg) and left eye (OS) = 11.1 ± 2.3 mm Hg (8–21 mm Hg). Median intraocular pressure for flamingos with the head in an upright position was OD and OS = 11 mm Hg. Mean intraocular pressure for flamingos with the head in a feeding position was OD = 14.3 ± 2.5 mm Hg (10–22 mm Hg) and OS = 14.4 ± 2.7 mm Hg (11–24 mm Hg), which were significantly higher. Median intraocular pressure for flamingos with the head in a feeding position was OD and OS = 14 mm Hg.