Blood samples of 86 captive panther chameleons (Furcifer pardalis) were collected in January and August from the ventral coccygeal vein in order to establish reference intervals of clinical healthy individuals under similar husbandry conditions for plasma biochemistry and hematology for this species. Significant differences were found in phosphorus, glucose, total protein, albumin, and white blood cell count between males and females in both seasons. Calcium, alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase varied in only one season between genders. Significant differences between summer and winter values were present in both genders for uric acid, calcium, phosphorus, glucose, total protein, creatine kinase, and albumin. Additionally, females showed seasonal variations for alanine aminotransferase and aspartate aminotransferase whereas packed cell volume varied in males. Gravid females had significantly higher body weights and increased values for uric acid, calcium, phosphorus, alanine aminotransferase, total protein, and albumin. Cytomorphologic characteristics of blood cells in stained blood films were evaluated to serve as additional parameters for hematology.