Computed tomography (CT)–guided percarapacial ovocentesis in an Eastern box turtle (Terrapene carolina) was performed after medical management failed to resolve dystocia related to an irregular, oversized egg (38 × 30 × 35 mm). A 2.38-mm Steinmann pin was drilled through the carapace at the dorsal aspect of the seventh marginal scute, allowing introduction of an 18-ga needle through the carapace and oviduct into the egg using CT guidance. Contents of the egg (8 ml) were evacuated and CT reconstructions confirmed involution. The animal was administered oxytocin immediately after the procedure and passed the collapsed egg within 3 hr. The animal displayed no adverse effects from the procedure and the carapacial wound healed quickly without complication. CT-guided needle aspiration and biopsy techniques are common in human medicine, but less frequently utilized in veterinary medicine. This technique represents a novel, minimally invasive approach to ovocentesis that was used as an alternative to surgical intervention, in this case to resolve dystocia.