Thirteen wild-caught hellbenders (Cryptobranchus alleganiensis) were treated for Batrachochytrium dendrobatidis with continuous chloramphenicol baths at concentrations of 20 mg/L for 14 days and 200 mg/L for 14 days. Clinical signs and deaths continued after treatment with 20 mg/L chloramphenicol but ceased after treatment with 200 mg/L chloramphenicol. No evidence of toxicity was found on hematologic tests, necropsy, or histopathologic examination. Lower infection burdens were detected after treatment with chloramphenicol, but infection rates were unchanged. Chloramphenicol may be useful as a treatment for B. dendrobatidis in hellbenders but did not clear hellbenders of infection.