Translator Disclaimer
16 April 2021 Genetic Assessment on the Origin of Alien Macaques in the Boso Peninsula in Japan
Yoshi Kawamoto
Author Affiliations +
Abstract

Japanese macaques and alien macaques have hybridized in the Boso Peninsula, Chiba Prefecture, Japan. In this study, the origin of the alien species was investigated by molecular assessments with mitochondrial DNA (mtDNA) and Y-chromosome genes. Maternal origin was assessed by comparing mtDNA sequence records. The results suggested that the alien species in the southern part of peninsula originated from the rhesus macaques in eastern China. Y-chromosome assessments with three microsatellite (Y-STR) loci detected a unique haplotype that is distributed in Japanese macaque habitats. Its origin was assessed by the TSPY (testis-specific protein on Y-chromosome) gene, suggesting the possibility of the involvement of long-tailed macaques in the Indochina region or rhesus macaques different from known source. Further investigation of historical documents and interviews disclosed the existence of a facility of long-tailed macaques planned for vaccine production in the past. This study presented novel evidence that the hybridization of Japanese macaques in the Boso Peninsula has the possibility to associate not only with rhesus macaques, but also with long-tailed macaques from the Indochina region. It is important to further monitor the status of Japanese macaques and changes in their hybridization in the peninsula for future conservation purposes.

In accordance with international trends aimed at comprehensively conserving biodiversity and using biological resources sustainably, the Japanese government enacted the Invasive Alien Species Act in 2005 to prevent damages from invasive alien species (Mito and Uesugi 2004). The objectives of this act are to regulate various actions, such as raising, planting, storing, carrying, and importing invasive alien species (IAS) in addition to mitigating the IAS that already exist in Japan, and prevent damages against biodiversity, human safety, or agriculture in Japan (Japanese Ministry of the Environments 2004). The act has been expanded to cover hybrids by an amendment in 2013. The most serious concern of the alien species issue on Japanese macaques (Macaca fuscata), an endemic non-human primate in Japan, is impacted by hybridization, which will destroy species biodiversity. The primates designated as specific alien species so far in the act are three macaques (rhesus macaques M. mulatta, Taiwanese macaques M. cyclopis, and long-tailed macaques M. fascicularis) and two of their hybrids (hybrids with M. mulatta and hybrids with M. cyclopis).

Rhesus macaques, which have become feral and hybridized with Japanese macaques, live in the southern tip of the Boso Peninsula in Honshu, Japan (Fig. 1). This species is naturally distributed across the Asian continent, from Afghanistan in the west, to China in the east (Fooden 2000). Although the history of their introduction to Boso peninsula is less known, it likely happened in the 1950s when monkeys became popular in the tourism industry and were later released when the business was abandoned (Hagihara et al. 2003). Based on the national policy, Chiba Prefecture began their eradication plan from 2007 (Chiba Prefecture 2012). The plan was revised in 2012 and the hybrid population is being reduced by euthanasia. Their population size was estimated to be 600–700 in 2012 (Chiba Prefecture 2012).

Fig. 1.

Map showing the location of habitats of indigenous Japanese macaques (in 2015, Chiba Prefecture 2017) and introduced rhesus macaques (year unspecified, Chiba Prefecture 2012) in the Boso Peninsula, Chiba Prefecture, Japan. Approximate distribution areas of groups are shown in color. The town name in parentheses indicates the town that existed before the merger of municipalities.

fi_ms2020-0078_001.jpg

The native Japanese macaque is distributed in the central hilly area, and there is an empty area of approximately 20 km, separating them from the habitat of rhesus macaques (Fig. 1). The geographical distribution of Japanese macaque groups was confirmed for the first time in 1923 (Iwano 1974). The existence of alien macaque groups and their habitat information were firstly reported in 1996 (Chiba Prefecture and Boso Monkey Management Study Group 1996). Migrations of solitary males were reported between these two species habitats (Hagihara et al. 2003). The Japanese macaque habitat extends to the south, north, and east due to recent population increases. Their habitat spans ten municipalities and was estimated to be 747 square kilometers in 2015 (Chiba Prefecture 2017).

Macaques form a population of matrilineal groups that make up social units, and the contrast between female philopatry and male dispersal is known in the life history of males and females (Pusey and Packer 1987; Melnick et al. 1992). It is known that sex-specific genes of mitochondrial DNA (mtDNA) and Y-chromosome DNA, which reflect this socio-ecological characteristic, are effective for population surveys as molecular markers for interspecific hybridization (Tosi et al. 2002).

Government officials and researchers have reported hybridization caused by the alien macaques on the Boso Peninsula (Kawamoto et al. 2004, 2007, 2017). Kawamoto et al. (2004) firstly reported hybridization in the feral rhesus monkey group and hybridization in the Japanese macaque group. After that, the hybridization status of the rhesus group was further investigated, and the results forewarned that the impact of the alien species could spread broadly to Japanese macaque population on the peninsula (Kawamoto et al. 2007). In the population monitoring of these surveys, hybrid individuals were identified by inspection of morphological characteristics such as relative tail length and coat color (Hamada 2013), and genetic examination using nuclear markers such as blood protein electromorphs (adenosine deaminase and NADH-dependent diaphorase) that specifically distinguish Japanese macaques from other Asian macaques (Nozawa et al. 1977; Kawamoto et al. 2004, 2007). Maternally inherited mtDNA has been used in this monitoring as a molecular marker for determining the natal place of monkeys, where the difference between Japanese macaques on the peninsula and the feral rhesus macaques in southern Boso area were examined from the difference in PCR product sizes or their sequence differences (Hagihara et al. 2003). It is confirmed that macaque populations on the peninsula are hybridizing in their habitats. However, information is limited regarding their place of origin and sufficient consideration has not been given to the possibility of the involvement of species other than rhesus macaques.

In this study, the origin of alien macaques was investigated with sex-specific markers of mtDNA and Y-chromosome DNA. DNA sequencing and fragment analyses were performed for species diagnostics. The possibility of alien species and its origin was finally assessed from molecular phylogenetic analysis using the available sequence information of macaques registered in the DNA database. For alien species newly hypothesized based on the obtained results, written records, and interviews with locals further examined the potential involvement in hybridization with Japanese macaque populations in the suspicious areas.

Materials and methods

Molecular markers

Mitochondrial DNA (mtDNA) and Y-chromosome genes were used to identify the species involved in hybridization. Genetic markers were transmitted in a sex-specific manner for females and males, and lots of sequence information were available for them. Due to its high evolutionary rate, mtDNA is the most popular marker in studies on evolution, phylogeny, and ecology. Information on rhesus macaques initially increased for monkeys used in the biomedical sciences and has lately been augmented for wild populations. On the other hand, due to its low evolutionary rate, the Y-chromosome gene is generally substandard as a marker. In evolutionary research on primates, using variations of the TSPY (testis-specific protein on Y-chromosome) gene (Kim et al. 1996; Tosi et al. 2000) has gradually increased and related information has recently been enriched. In addition, microsatellite DNA markers for Y-chromosomes (Y-STR) initially developed in human genetics have been applied to non-human primates (for example, Erler et al. 2004; Kawamoto et al. 2008).

Two kinds of molecular markers, Y-STR and TSPY, were adopted to evaluate the origin of Y-chromosome DNA in this study. At first, haplotypes of Y-chromosome were classified by fragment analyses of three Y-STR loci. From this haplotyping, the distribution area and haplotype frequencies were summarized to see fundamental differences of Y-chromosome composition between Japanese macaque populations in the central hilly area and rhesus population in southern Boso area. Two different sampling periods (before May 2009 and during March 2011–January 2018) were compared to evaluate changes in Y haplotype composition. For the rhesus habitats in Tateyama city and Minamiboso city (separated into Shirahama and Tomiyama towns before the merger of municipalities in March 2006), male migrants from Japanese macaque groups were judged based on features of morphology and mtDNA types. For the Japanese macaque habitats, the Y haplotypes of hybrid individuals were evaluated based on morphological features of relative tail length and coat color and genotyping results of protein-coding loci of adenosine deaminase and NADH-diaphorase. From the screening of Y haplotypes, three different representatives of Y-chromosomes, each for the Japanese macaque, the rhesus macaque, and the other one that could be assigned to neither species, were selected, then they were subjected to the sequencing of the TSPY gene to assess species origin.

Study area and samples

Samples of blood, tissue, or fecal DNA were collected from the Boso Peninsula (Fig. 1, Table 1,  Supplementary Table S1 (08kawamoto_2020-0078_TS1.pdf)). Blood samples were taken from captured individuals within the scope of the prefecture's program. Clotting was prevented with sodium heparin or EDTA, and cryopreserved blood cells were used in experiments. Tissue samples of ear skin collected from monkeys exterminated by the government's pest control project were also used in this study. Tissue DNA was extracted using conventional Phenol/Chloroform/Isoamyl alcohol and ethanol precipitation methods (Sambrook et al. 1989 with slight modifications). Fecal samples were collected in the Japanese macaque habitat following the procedure of a previous study (Hayaishi and Kawamoto 2006). Fecal DNA samples were prepared following the procedure of Kawamoto et al. (2013).

Sampling was performed with official permission given in the prefectural plan, and the handling of animals and sampling procedures followed the guidelines of the Primate Research Institute, Kyoto University (Guideline for Field Research of Non-human Primates) and that of the Mammal Society of Japan (Guidelines for the Procedure of Obtaining Mammal Specimens).

Table 1.

List of samples examined

ta_ms2020-0078_001.gif

DNA analysis

Direct sequencing of the non-coding region was done with mtDNA typing. An amplicon from blood, tissue DNA, or fecal DNA was subjected to direct sequencing, where the PCR reaction mixture (25 µl) contained 1 µl of template DNA, 12.5 µl of 2 × buffer, 0.4 mM of each dNTP, 300 nM of each of the primers, LqqF (forward) 5′-TCCTAGGGCAATCAGAAAGAAAG-3′ (Li and Zhang 2004) (corresponding to nucleotides 15936–15958 of a complete mtDNA sequence of Japanese macaques, accession no. NC_025513 in DDBJ/ENA/GenBank databases) and Saru5 (reverse) 5′-GGCCAGGACCAAGCCTATTT-3′ (Hayasaka et al. 1991) (nucleotides 609–628, NC_025513), and 0.5 U of DNA polymerase KOD-FX (Toyobo, Osaka, Japan). The thermal cycling condition involved initial heat denaturation at 94°C for 2 min, followed by 35–45 cycles of denaturation at 98°C for 10 sec, annealing at 58°C for 30 sec and extension at 68°C for 30 sec. Four additional internal primers were also used for sequencing; 53F (forward) 5′-CTCACCATCCTCCGTGAAAT-3′ (nucleotides 16393–16412, NC_025513), Saru4 (forward) 5′-ATCACGGGTCTATCACCCTA-3′ (nucleotides 2–21, NC_025513), 51R (reverse) 5′-CATGGAAAGCTCCCGTGACT-3′ (nucleotides 28–47, NC_025513), and mdl341 (reverse) 5′-GTTTGGATGAAGGTCGGAGA-3′ (nucleotides 315–324, NC_ 025513). Sequencing was performed with an ABI 3130xl Genetic Analyzer (Applied Biosystems, CA, USA).

TSPY direct sequencing was conducted similarly to the method of mtDNA sequencing, where primers TSPY-A (forward) and TSPY5R (reverse) were used to obtain a PCR amplicon, and internal primers 470F (forward), 485R (reverse), and 740R (reverse) were used to obtain sequence reads (Tosi et al. 2000). Y-STR analysis was done with three STR markers on Y-chromosomes, DYS472, DYS569, and DYS645 (Kawamoto et al. 2008). Haplotypes were classified from combinations of allele types. The fragment analysis condition followed the procedure of Kawamoto et al. (2008). Allele sizes were determined using GeneMapper v. 4.1 (Applied Biosystems).

The DNA sequences obtained in this study were registered in DDBJ/ENA/GenBank databases under accession numbers LC585811–LC585868.

Data analysis

DNA sequences were verified with Sequence Navigator (Applied Biosystems). Sequences of mtDNA or TSPY genes were compared to reference sequences in the database as listed in Tables 2 and 3. Multiple sequence alignments were taken with ClustalX (ver. 2.0) (Thompson et al. 1997) after selecting conserved blocks using Gblocks version 0.91b (Castresana 2000). Species origin was verified by evaluating the phylogenetic relationships of sequences using programs in MEGA6 (Tamura et al. 2013).

Table 2.

List of reference rhesus sequences and their accession numbers for mtDNA assessment

ta_ms2020-0078_002.gif

Unique Y haplotypes were classified in the Y-STR analysis. A total of 261 males were grouped in the municipality origin. Then the distribution of haplotypes was compared to explore which species were involved. In order to evaluate the relationship among Y haplotypes classified by the STR analyses, an unrooted tree diagram was constructed assuming a stepwise mutation model (Kimura and Ohta 1978). Here, the genetic distance between Y haplotypes was simply defined as the sum of the differences in the number of repetitive units over the three Y-STR loci. The tree diagram was generated from the obtained distance matrix using the programs Neighbor and Drawtree implemented in the software package PHYLIP (Felsenstein 1989). The TSPY sequences of three unique Y-STR haplotypes were finally compared with reference TSPY data to find relevant species and their geographic origin.

Table 3.

List of reference macaque sequences and their GenBank accession numbers for TSPY gene assessment

ta_ms2020-0078_003.gif

Fig. 2.

Comparison of mtDNA sequences by alignments of three Japanese types (Mf-A, Mf-B, and Mf-C) and one rhesus type (Mm) detected in Boso, Chiba. Nucleotide location in this figure refers to the nucleotide position (NP) number of a complete mtDNA sequence of rhesus macaques (GenBank accession no. KF830702). Gray letters indicate corresponding sites in the reference KF830702 without variation in this study.

fi_ms2020-0078_002.jpg

Results

Assessment using mtDNA

Differences among species were clearly found in mtDNA by sequencing 1007–1011 bps. There were size differences in the amplicons. An insertion of five bases and a deletion of a single base were detected in Japanese macaques compared to the exotic sequence (Fig. 2). In 47 samples (28 blood samples, two tissue samples, and 17 feces) typed as Japanese macaques, three mtDNA haplotypes (Mf-A, Mf-B, and Mf-C types in Fig. 2) were distinguished due to base substitutions. On the other hand, all of the examined samples from the alien habitat in southern Boso showed the same sequence (Mm type in Fig. 2).

The exotic mtDNA sequence in southern Boso was preliminarily evaluated to be a rhesus type by BLAST (Basic Local Alignment Search, a sequence homology search tool supported by NCBI, USA). It was further compared to 409 reference sequences (Table 2) to investigate the place of origin. The final length of the compared sequence was only 445 bps after Gblocks sorting due to available information. As a result, 223 mtDNA haplotypes were distinguished. There was one registered record (accession number AY646959) completely identical to the exotic type. It was a record on a rhesus monkey imported from China to USA (Smith and McDonough 2005). Figure 3 is a neighbor-joining tree (Saitou and Nei 1987) drawn from the 223 haplotypes. The contents of those haplotypes are summarized in  Supplementary Table S2 (08kawamoto_2020-0078_TS2.pdf). From this result, it was concluded that the rhesus macaques in Boso did not originate from South Asia (India, Bangladesh, and Nepal) and Southeast Asia (Myanmar and Thailand). When inspecting their origin and vicinity in China, it was not easy to point a specific place. Composed of sub-clusters, the Boso rhesus type was clustered together with those from Anhui, Fujian, Guangdong, Guangxi, Hubei, and Zhejiang Provinces and an unknown area in eastern China (Fig. 3).

Fig. 3.

A neighbor-joining tree drawn to find the origin of rhesus macaque type in Boso, where 223 haplotypes defined from 410 records in the database by matching sorted 445 bps in mtDNA D-loop were compared to the Boso type. The default parameters were set when the tree was constructed using programs in MEGA6 (Tamura et al. 2013). The reference haplotype data were categorized by locality origin. The haplotypes of Bangladesh and Vietnam, which showed diversity and were scattered in the tree, are represented by separate symbols. For closely related haplotypes, the Boso type was compared in a separate box. The haplotypes that make up the haplogroups A to G are summarized in Supplementary Information. Abbreviations for Chinese locality are given as NW = northwest, SW = southwest, W = west, E = east, and S = south by taking descriptions of Smith and McDonough (2005) and Satkoski et al. (2008) into consideration.

fi_ms2020-0078_003.jpg

Assessment using Y-chromosome DNA

Table 4 shows the results of the Y-STR analysis. A total of eight Y haplotypes were distinguished by Y-STR polymorphisms. The repetitive units of the DYS472, DYS569, and DYS645 markers were three bases, four bases, and one base, respectively, and the number of alleles detected for DYS472, DYS569, and DYS645 was six, four, and five, respectively.

Table 4.

Summary of Y chromosome haplotypes defined by Y-STR markers (DYS472, DYS569, and DYS645)

ta_ms2020-0078_004.gif

Fig. 4.

An unrooted tree diagram showing the relationship among eight Y haplotypes distinguished by Y-STR analyses. Abbreviation name and allelic profile of each Y haplotype are summarized in Table 4. The genetic distances between Y haplotypes were measured with the total number of repetitive units (unit distance). The tree diagram was drawn from an outfile of UPGMA tree given by Neighbor program using Drawtree program in PHYLIP package (Felsenstein 1989).

fi_ms2020-0078_004.jpg

The relationship among the Y haplotypes was evaluated with an unrooted tree diagram as shown in Fig. 4. The three Japanese haplotypes (J1, J2, and J3) were closely clustered, but the other haplotypes were distantly related to the Japanese group. Two groups, one consisting of R2, R4, and U and the other of R1 and R3, were observed in the tree diagram as non-Japanese Y haplogroups.

Six haplotypes; J1, J2, J3, R1, R4, and U were detected in the Japanese macaque habitat. Six haplotypes of R1, R2, R3, R4, J1, and J2 were detected in the rhesus macaque habitat. J1, J2, and J3 were judged to be the Y-chromosome types derived from Japanese macaques, and R1, R2, R3, and R4 were judged to be the Y-chromosome types derived from rhesus macaques, based on the area of occurrence, frequency, and the STR allele types constituting the Y haplotypes (Table 4 and Fig. 4). The U type was detected in a small part of the eastern and northern area of the Japanese macaque habitat, but not in the rhesus macaque area. However, the ten individuals having this type showed more or less different characteristics in the relative tail length and coat color morphology, and many of them had blood protein variations of macaques other than Japanese macaques ( Supplementary Table S3 (08kawamoto_2020-0078_TS3.pdf)). Therefore, this Y haplotype was separately denoted as an un-assignable type (abbreviated as U type) to distinguish it from the Japanese macaque type, and its carrier was regarded as a hybrid with an unknown alien macaque species.

Upon comparing samples that had been collected until May 2009 when the hybridization was less advanced, a total of eight unique Y haplotypes, three Japanese types, four rhesus types, and one un-assignable type (U type), were distinguished from an examination of 151 male blood samples collected in 11 administrative districts (the municipalities at the time) (Table 4). Two Japanese types and four rhesus types were detected in Tateyama city and Shirahama town (currently Minamiboso city) in the rhesus habitat. On the other hand, only the Japanese macaque types, excluding the two cases of R4 type (Kyonan town), were found in all eight administrative districts of the Japanese macaque habitat. The U type was detected only in Kisarazu city and Ichihara city at that period. When comparing the results of the 111 males collected in the Japanese macaque habitat from March 2013 to January 2018, a total of eight U type individuals were detected in multiple groups in Katsuura city (Table 4).

The TSPY gene sequences were compared to ascertain the origin of the U type, which was uncertain from the Y-STR analysis. For a representative comparison, samples of Japanese macaques (Kyonan town), rhesus macaques (Minamiboso city), and the U type (Katsuura city) were sequenced for 1491 bps and compared with deposited database of rhesus macaques, Taiwanese macaques, long-tailed macaques, and pig-tailed macaques (M. nemestrina) (Fig. 5). In the constructed tree, the U type was clustered together with rhesus macaque types from India and China and a long-tailed macaque type from Vietnam. The rhesus type in Boso was also placed in the same cluster. The Japanese type in Boso formed a cluster together with the registered types of Japanese macaques. No sequence differences were detected among eight individuals of the U type from Katsuura city in Table 4.

Discussion

The DNA markers adopted in this study contrast in the practices of female philopatry and male dispersal (Pusey and Packer 1987; Melnick et al. 1992). The strong philopatric features of females make mtDNA dispersal restricted from natal groups. Males can geographically disperse mtDNA but cannot transmit it due to its maternal mode of inheritance. Therefore, the dispersal lifetime of mtDNA by males is limited within one generation. Meanwhile, chromosomal pairing is cytogenetically restricted, and the recombination portion is small between the Y-chromosome and X-chromosome in macaques (Hirai et al. 1991). Although its mutation rate is not as high as mtDNA, the non-recombined part of Y-chromosome can simply accumulate mutations like mtDNA. Due to these characteristics, both mtDNA and Y-chromosomes were regarded as suitable tools to assess the origin of alien species in this study. Like autosomal chromosomes, Y-chromosomes are transmitted across generations. Thus, interspecific introgression can be thought to begin through male migration, and the impacts of alien species can spread geographically by succeeding male dispersal in later generations.

Fig. 5.

A neighbor-joining tree constructed from representative sequences of TSPY gene by matching sorted 1491 bps (Table 3). The default parameters were set when the tree was constructed using programs in MEGA6 (Tamura et al. 2013). Three types of TSPY sequences detected in the present study, including the U type defined by Y-STR haplotyping, were compared with reference sequences of macaque species. The values above the branch node indicate the results of interior-branch test (in non-italic) and the percentage bootstrap values (in italic) obtained from assessments with the NJ algorithm (1000 replications) (Sitnikova et al. 1995).

fi_ms2020-0078_005.jpg

In the rhesus population of southern Boso, there was only one type of mtDNA, but multiple Y-haplotypes were detected. High maternal homogeneity suggests a monophyletic origin of the population, and the diversity of the Y haplotypes implies involvement of multiple males in founding.

Regarding the results of the mtDNA assessment, a preliminary study by Hagihara et al. (2003) speculated its origin from China or its vicinity by the examination of captive rhesus specimens collected at the Primate Research Institute, Kyoto University in Japan. The present study further compared their origin with data on captive rhesus macaques imported to the Regional Primate Centers in the USA, as well as data on wild individuals in the source countries. An earlier investigation by Smith and McDonough (2005) classified Chinese rhesus types into four groups, consisting of one group from the eastern region (denoted as “ChiE haplogroup” in Smith and McDonough (2005), corresponding to “China E” in Fig. 3) and three groups from the western region (denoted as “ChiW1, ChiW2, and ChiW3 haplogroups” in Smith and McDonough (2005), corresponding to “China W” in Fig. 3), where the Boso rhesus type matched completely with a haplotype in the “ChiE haplogroup” (accession number AY646959; Haplotype 47 in  Supplementary Table S2 (08kawamoto_2020-0078_TS2.pdf)). There were regional variations in mtDNA haplotypes that were close to the rhesus type of Boso in Fig. 3. Most of them were wild or captive rhesus macaques from provinces in eastern China. However, the cluster also contained haplotypes from breeding centers in south-central or southeast China. Satkoski et al. (2008) pointed out that there was anthropogenic activity in Chinese breeding colonies that resulted in the export of hybrids. Considering this influence, it may be reasonable to conclude that the origin of rhesus populations in southern Boso is somewhere in east China, probably Anhui, Fujian, Zhejiang, or Jiangsu Province. If hybridization in the Boso Peninsula cannot be prevented, the species biodiversity of the Japanese macaque will be significantly affected by mixing with the rhesus macaque from east China.

This study presented new results for understanding the history of the macaque hybridization on the Boso peninsula. It is noteworthy that the U type of the Y-chromosome haplotype was found only in a restricted habitat of Japanese macaques and was not observed in the rhesus area of southern Boso. There were no groups of Japanese macaques in the U type area in 1973 before their habitat expansion (Boso Japanese Monkey Research Group 1979). The individuals carrying the U type contained many hybrids in the inspection of morphological traits and diagnostic autosomal protein markers ( Supplementary Table S3 (08kawamoto_2020-0078_TS3.pdf)). The Japanese macaque groups in Katsuura city has been recently established by population expansion, and their habitat is located farther from the rhesus distribution area than the Japanese macaques in the western part of the peninsula. As the rhesus specific Y-chromosome types R1–R4 have not been observed in Katsuura (Table 4), it is also inferable that the influence of gene flow from the rhesus population in southern Boso may be low in this area. These circumstantial findings imply the involvement of alien macaques other than the rhesus macaques in southern Boso. Unfortunately, the details of morphological features of the U type carrier are unknown due to the paucity of records on captured individuals. It has been discussed that rhesus population in southern Boso is the cause of hybridization with Japanese macaques on the Boso Peninsula, but based on the obtained results in this study, it is now necessary to investigate further the cause(s) of hybridization. Hagihara et al. (2003) reported alien macaques listed in the administrative statistics and interviewing records to leisure facilities at that moment, but there were no records of alien species that seemed to be related to the distribution of the U carrier.

Three reference data of rhesus and long-tailed macaques showed the same sequence with the TSPY sequence of U type (Fig. 5), as well as the sequence of rhesus in southern Boso. The U sequence matched with one of the two clusters of long-tailed macaques of Indochina origin (Vietnam), but not a Sundaic origin (Malaysia, Indonesia, and the Philippines). Though this result could not exclusively conclude the origin of the U type from long-tailed macaques, its possibility was supported as well as that of rhesus origin. Studies on the evolution and phylogeny of macaques in Southeast Asia revealed ancient introgression between rhesus macaques and long-tailed macaques in the region around 15 degrees north latitude of the Indochina Peninsula (Tosi et al. 2002; Hamada et al. 2006; Street et al. 2007; Bonhomme et al. 2009; Barr et al. 2011, Jadejaroen et al. 2015; Bunlungsup et al. 2017b). The populations of long-tailed macaques in the region share the Y-chromosomes with rhesus macaques due to this evolutionary history. Thus, the result of the Y-chromosome assessment in this study suggests that long-tailed macaques from Indochina may have been involved in hybridization. As an alternative case, if long-tailed macaques are not involved, a rhesus population different from that in southern Boso could be considered as the cause of hybridization because of absence of the U haplotype in southern Boso.

As the males with the U type had a Japanese mtDNA haplotype (for males in Katsuura city, see note in  Supplementary Table S1 (08kawamoto_2020-0078_TS1.pdf)), it may be reasonable to consider the Y gene flow by immigrant males. To explain this contrast between Y DNA type and mtDNA type, a hypothesis was considered that there had been extinction or removal of source alien macaque population(s) in the survey area of this study. One way to further test this hypothesis was to discover records of foreign macaque introduction or find persons who knew about the history of introduction in the study area.

The incident of anthropogenic introduction was further investigated through a survey of historical documents and interviews with people who knew the past. Owing to many collaborators, it became clear that there was a national project for the production of the polio vaccine in the Boso Peninsula around 1960. The project, which began in 1958, aimed at developing the Salk vaccine (Francis et al. 1955; Meldrum 1998) that could be produced by inactivating the virus propagated in a culture of monkey kidney cells. It was confirmed that a facility for breeding long-tailed macaques was established in the project, firstly at Sakura city in 1959, and was then relocated to Katsuura city in 1960 (Chiba Serum Institute 1977, 1997; Katsuura city 1961) (Fig. 1). The breeding colony was converted into a tourist facility in the late 1960s when changes in vaccine production made it unnecessary to maintain it (Chiba Serum Institute 1997). Finally, the facility was closed in 1984 by removing all the monkeys from the place. The population of long-tailed macaques had been kept at the tip of Hachiman-misaki (Fig. 1) in free-ranging conditions by the city's provisioning efforts (Fig. 6), and there were 230 individuals at most in 1964 (Katsuura city 1964). The signboard at that time stated that the origin of the monkeys was the Malay Peninsula (Chiba Serum Institute 1997). However, when we checked a report on trade statistics (Kawanishi and Honjo 1971), little evidence was given to support the number of imports from the Malay Peninsula at that time. In addition, the sequence of U individuals did not match the TSPY gene sequences of long-tailed macaques in the Malay Peninsula provided by the Wildlife Genetic Resource Bank (WGRB) Laboratory of the Malaysian government (GenBank accession numbers KJ690361–KJ690376). Therefore, given the impact of imported long-tailed macaques from the polio vaccine project, it can be inferred that their origin was not in the Malay Peninsula.

Fig. 6.

Photographic record of a group of long-tailed macaques provisioned at the tip of Hachiman-misaki, Katsuura city in April 1981 (courtesy of Mr. Satoshi Inoue).

fi_ms2020-0078_006.jpg

Long-tailed macaques may have dispersed into the surrounding area over the past 25 years. In the early stage, there was no group of Japanese macaques in the surrounding area, but subsequent increased encounters between the two species might have triggered hybridization. Since the transplanted long-tailed group finally was removed from the leisure facility, this hybridization impact might have remained in the form of unidirectional gene flow to Japanese macaques. As there is no available specimen or sample of the long-tailed macaque at present, it is hard to provide more scientific evidence to support this speculation.

In conclusion, this study suggests that the ongoing hybridization of Japanese macaques on the Boso Peninsula may be associated not only with rhesus macaques, which came from eastern China, but also with long-tailed macaques from the Indochina region. Keeping the findings of this assessment in mind, it is important to further monitor the status and change of hybridization on the Boso Peninsula and use the outcomes to establish countermeasures to conserve Japanese macaques in the future.

Supplementary data

Supplementary data are available at Mammal Study online.

 Supplementary Table S1 (08kawamoto_2020-0078_TS1.pdf). Information on the samples used in mtDNA examination.

 Supplementary Table S2 (08kawamoto_2020-0078_TS2.pdf). List of mtDNA 223 haplotypes classified by multiple alignments of 409 D-loop reference data deposited in the GenBank database.

 Supplementary Table S3 (08kawamoto_2020-0078_TS3.pdf). Results of hybrid judgement for males that carried the U type of Y chromosome in Table 4.

Acknowledgments:

I am indebted to the people and administrative agencies involved in the alien species control project in Chiba Prefecture. The Chiba Prefecture Nature Conservation Division and Futtsu City Commerce and Industry Tourism Division cooperated in providing samples and related information. I am deeply grateful to Mr. Kei Shirai, Mr. Yoji Naoi, Mr. Ko Hagihara, Mr. Daisuke Shiratori, Mr. Fumitaka Ikeda, Mr. Keigo Aizawa, Mr. Misao Okano, Mr. Yoshifumi Sugiura, Mr. Tatsuaki Kondo, Ms. Aki Kawamura, Dr. Yuzuru Hamada, and Dr. Tsuyoshi Ito for their field observations, sample collections, and information on individual samples. Dr. Hisashi Yamakawa, Dr. Hiroyuki Tanaka, Dr. Yasuhiro Go, Dr. Shoji Tatsumoto, Dr. Hironaga Kakoi, and Ms. Sakie Kawamoto provided valuable ideas and supported the genetic analyses. I would also like to thank Dr. Sayaka Shimoinaba, Mr. Tei Shimizu, and Mr. Satoshi Inoue for the survey of historical data on alien species. I also express my gratitude to Dr. Shinichi Hayama, Dr. Tamaki Maruhashi, and Dr. Yoshiki Morimitsu for their encouragement and advice on this study. I would like to thank Dr. Michael Huffman for the English proofreading of the manuscript.

This report was prepared based on an invitation by Dr. Hiroto Enari and Dr. Yamato Tsuji, who were the organizers of a cooperative research meeting of the Primate Research Institute, Kyoto University, held on February 8, 2020. Part of this study was financially supported by the 2019 Cooperative Research Program of the Primate Research Institute, Kyoto University (No. 2019-B-52, collaborated with Dr. Hiroyuki Tanaka).

References

1.

Barr, A., Premasuthan, A., Satkoski, J., Smith, D. G., George, D. and Kanthaswamy, S. 2011. A rapid quantitative real-time PCR-based DNA quantification assay coupled with species-assignment capabilities for two hybridizing Macaca species. Folia Primatologica 82: 71–80. Google Scholar

2.

Bonhomme, M., Cuartero, S., Blancher, A. and Crouau-Roy, B. 2009. Assessing natural introgression in 2 biomedical model species, the rhesus macaque (Macaca mulatta) and the long-tailed macaque (Macaca fascicularis). Journal of Heredity 100: 158–169. Google Scholar

3.

Boso Japanese Monkey Research Group. 1979. List of vegetable foods of Japanese macaques in the Boso hills: Addendum. Kiyosumi 7: 31–38 (in Japanese). Google Scholar

4.

Bunlungsup, S., Imai, H., Hamada, Y., Matsudaira, K. and Malaivijitnond, S. 2017a. Mitochondrial DNA and two Y-chromosome genes of common long-tailed macaques (Macaca fascicularis fascicularis) throughout Thailand and vicinity. American Journal of Primatology 79: 1–13. Google Scholar

5.

Bunlungsup, S., Kanthaswamy, S., Oldt, R. F., Smith, D. G., Houghton, P., Hamada, Y. and Malaivijitnond, S. 2017b. Genetic analysis of samples from wild populations opens new perspectives on hybridization between long-tailed (Macaca fascicularis) and rhesus macaques (Macaca mulatta). American Journal of Primatology 2017: e22726. Google Scholar

6.

Castresana, J. 2000. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17: 540–552. Google Scholar

7.

Chiba Prefecture. 2012. Control Implementation Plan of Specific Alien Species (Rhesus Monkeys). Available at  https://www.pref.chiba.lg.jp/shizen/choujuu/akagezaru/documents/akagekeikakuh23kai.pdf (Accessed 17 January 2021) (in Japanese). Google Scholar

8.

Chiba Prefecture. 2017. The Fourth Specified Wildlife (Class Two) Conservation and Management Plan for Japanese Monkeys. Available at  https://www.pref.chiba.lg.jp/shizen/choujuu/jigyoukeikaku/documents/4-saru-keikaku.pdf (Accessed 17 January 2021) (in Japanese). Google Scholar

9.

Chiba Prefecture and Boso Monkey Management Study Group. 1996. Project Research Report on Management Measures Survey of Wild Monkeys on Boso Peninsula in Fiscal Year 1995: 14–17 (in Japanese). Google Scholar

10.

Chiba Serum Institute. 1977. History of Chiba Serum Institute: 30th anniversary. Chiba Serum Institute, Ichikawa, 155 pp. (in Japanese). Google Scholar

11.

Chiba Serum Institute. 1997. Chiba Serum Institute 50 Years History. Chiba Serum Institute, Ichikawa, 207 pp. (in Japanese). Google Scholar

12.

Erler, A., Stoneking, M. and Kayser, M. 2004. Development of Y-chromosomal microsatellite markers for nonhuman primates. Molecular Ecology 13: 2021–2930. Google Scholar

13.

Felsenstein, J. 1989. PHYLIP–Phylogeny inference package. Cladistics 5: 164–166. Google Scholar

14.

Fooden, J. 2000. Systematic review of the rhesus macaque, Macaca mulatta (Zimmermann, 1780). Fieldiana Zoology 96: 1–180. Google Scholar

15.

Francis, T. Jr., Korns, R. F., Voight, R. B., Boisen, M., Hemphill, F. M., Napier, J. A. and Tolchinsky, E. 1955. An evaluation of the 1954 poliomyelitis vaccine trials: summary report. American Journal of Public Health and the Nation's Health 45: 1–63. Google Scholar

16.

Hagihara, K., Aizawa, K., Kabaya, H. and Kawamoto, Y. 2003. Habitat status and genetic profile of the macaque populations containing alien species in the Bousou Peninsula. Primate Research 19: 229–241 (in Japanese with English summary). Google Scholar

17.

Hamada, Y. 2013. Judgment of hybrids of rhesus and Japanese macaques based on morphological indicators. Primate Research 29: 146–151 (in Japanese). Google Scholar

18.

Hamada, Y., Urasopon, N., Hadi, I. and Malaivijitnond, S. 2006. Body size and proportions and pelage color of free-ranging Macaca mulatta from a zone of hybridization in northeastern Thailand. International Journal of Primatology 27: 497–513. Google Scholar

19.

Hasan, M. K., Feeroz, M. M., Jones-Engel, L., Angel, G. A., Kanthaswamy, S. and Smith, D. G. 2014. Diversity and molecular phylogeny of mitochondrial DNA of rhesus macaques (Macaca mulatta) in Bangladesh. American Journal of Primatology 76: 1094–1104. Google Scholar

20.

Hayaishi, S. and Kawamoto, Y. 2006. Low genetic diversity and biased distribution of mitochondrial DNA haplotypes in the Japanese macaque (Macaca fuscata yakui) on Yakushima Island. Primates 47: 158–164. Google Scholar

21.

Hayasaka, K., Ishida, T. and Horai, S. 1991. Heteroplasmy and polymorphism in the major noncoding region of mitochondrial DNA in Japanese monkeys: Association with tandemly repeated sequences. Molecular Biology and Evolution 8: 399–415. Google Scholar

22.

Hirai, M., Terao, K., Cho, F. and Honjo, S. 1991. An XY/XYY mosaic cynomolgus monkey (Macaca fascicularis). Cytologia 56: 467–471. Google Scholar

23.

Iwano, T. 1974. Distribution of Japanese monkeys. The Nihonzaru 1: 5–62 (in Japanese). Google Scholar

24.

Jadejaroen, J., Hamada, Y., Kawamoto, Y. and Malaivijitnond, S. 2015. Use of photogrametry as a means to assess hybrids of rhesus (Macaca mulatta) and long-tailed (M. fascicularis) macaques. Primates 56: 77–88. Google Scholar

25.

Japanese Ministry of the Environments. 2004. Basic Policy for Preventing Adverse Effects on Ecosystems Caused by Invasive Alien Species (Cabinet Decision as of October 15, 2004). Available at  https://www.env.go.jp/en/nature/as/041108.pdf (Accessed 17 January 2021). Google Scholar

26.

Katsuura city. 1961. Katsuura City Handbook in 1961. 1961: 60, Katsuura City (in Japanese). Google Scholar

27.

Katsuura city. 1964. Katsuura City Handbook in 1964. 1964: 57, Katsuura City (in Japanese). Google Scholar

28.

Kawamoto, Y., Hagihara, K. and Aizawa, K. 2004. Finding of hybrid individuals between native Japanese macaques and introduced rhesus macaques in the Bousou Peninsula, Chiba, Japan. Primate Research 20: 89–95 (in Japanese). Google Scholar

29.

Kawamoto, Y., Kawamoto, S., Hamada, Y., Yamakawa, H., Naoi, Y., Hagihara, K., Shiratori, D., Shirai, K., Sugiura, Y., Go, Y., et al. 2017. Hybridization with rhesus macaques at Takagoyama Nature Zoo in the Boso Peninsula, Chiba Prefecture, and concern for expansion of hybridization to designated areas of natural monuments. Primate Research 33: 69–77 (in Japanese). Google Scholar

30.

Kawamoto, Y., Kawamoto, S., Kawai, S., Shirai, K., Yoshida, A., Hagihara, K., Shiratori, D. and Naoi, Y. 2007. Status report of hybridization in an introduced population of rhesus macaques (Macaca mulatta) in the Bousou Peninsula, Chiba, Japan. Primate Research 23: 81–89 (in Japanese). Google Scholar

31.

Kawamoto, Y., Takemoto, H., Higuchi, S., Sakamaki, T., Hart, J. A., Hart, T. B., Tokuyama, N., Reinartz, G. E., Guislain, P., Dupain, J., et al. 2013. Genetic structure of wild bonobo populations: Diversity of mitochondrial DNA and geographical distribution. PLOS One 8: e58660. https://doi.org/10.1371/journal.pone.0059660Google Scholar

32.

Kawamoto, Y., Tomari, K., Kawai, S. and Kawamoto, S. 2008. Genetics of the Shimokita macaque population suggest an ancient bottleneck. Primates 49: 32–40. Google Scholar

33.

Kawanishi, Y. and Honjo, S. 1971. Import situation of monkeys in Japan. Experimental Animals 20: 161–172 (in Japanese). Google Scholar

34.

Kim, H.-S., Hirai, H. and Takenaka, O. 1996. Molecular features of the TSPY gene of gibbons and Old World monkeys. Chromosome Research 4: 500–506. Google Scholar

35.

Kimura, M. and Ohta, T. 1978. Stepwise mutation model and distribution of allelic frequencies in a finite population. Proceedings of the National Academy of Sciences of the USA 75: 2868–2872. Google Scholar

36.

Kyes, R. C., Jones-Engel, L., Chalise, M. K., Engel, G., Heidrich, J., Grant, R., Bajimaya, S. S., McDonough, J., Smith, D. G. and Ferguson, B. 2006. Genetic characterization of rhesus macaques (Macaca mulatta) in Nepal. American Journal of Primatology 68: 445–455. Google Scholar

37.

Li, Q. and Zhang, Y. 2004. A molecular phylogeny of Macaca based on mitochondrial control region sequences. Zoological Research 25: 385–390 (in Chinese). Google Scholar

38.

Li, D. Y., Xu, H. L., Smith, D. G., Cheng, A. C., Trask, J. S., Zhu, Q., Yao, Y. F., Du, D. D. and Ni, Q. Y. 2011. Phylogenetic analysis of Chinese rhesus macaques (Macaca mulatta) based on mitochondrial control region sequences. American Journal of Primatology 73: 883–895. Google Scholar

39.

Meldrum, M. 1998. “A calculated risk”: the Salk polio vaccine field trials of 1954. British Medical Research (Clinical Research Edition) 317: 1233–1236. Google Scholar

40.

Melnick, D. J., Hoelzer, G. A. and Honeycutt, R. L. 1992. Mitochondrial DNA: its uses in anthropological research. In( Devor, E. J., ed.) Molecular Applications in Biological Anthropology, pp. 179–233. Cambridge University Press, Cambridge. Google Scholar

41.

Mito, T. and Uesugi, T. 2004. Invasive alien species in Japan: The status quo and the new regulation for prevention of their adverse effects. Global Environmental Research 8: 171–191. Google Scholar

42.

Nozawa, K., Shotake, T., Ohkura, Y. and Tanabe, Y. 1977. Genetic variations within and between species of Asian macaques. Japanese Journal of Genetics 52: 15–30. Google Scholar

43.

Pusey, A. E. and Packer, C. 1987. Dispersal and philopatry. In( Smuts, B. B., Cheney, D. L., Seyfarth, R. M., Wrangham, R. W. and Struhsaker, T. T., eds.) Primate Societies, pp. 250–266. University of Chicago Press, Chicago. Google Scholar

44.

Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425. Google Scholar

45.

Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1626 pp. Google Scholar

46.

Satkoski, J., George, D., Smith, D. G. and Kanthaswamy, S. 2008. Genetic characterization of wild and captive rhesus macaques in China. Journal of Medical Primatology 37: 67–80. Google Scholar

47.

Sitnikova, T., Rzhetsky, A. and Nei, M. 1995. Interior-branch and bootstrap tests of phylogenetic trees. Molecular Biology and Evolution 12: 319–333. Google Scholar

48.

Smith, D. G., George, D., Kanthaswamy, S. and McDonough, J. 2006. Identification of country of origin and admixture between Indian and Chinese rhesus macaques. International Journal of Primatology 27: 881–898. Google Scholar

49.

Smith, D. G. and McDonough, J. 2005. Mitochondrial DNA variation in Chinese and Indian rhesus macaques (Macaca mulatta). American Journal of Primatology 65: 1–25. Google Scholar

50.

Street, S. L., Kyes, R. C., Grant, R. and Ferguson, B. 2007. Single nucleotide polymorphisms (SNPs) are highly conserved in rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) macaques. BMC Genomics 8: 480. Google Scholar

51.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MAGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729. Google Scholar

52.

Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. 1997. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 1997; 24: 4876–2482. Google Scholar

53.

Tosi, A. J., Morales, J. C. and Melnick, D. J. 2000. Comparison of Y chromosome and mtDNA phylogenies leads to unique inferences of macaque evolutionary history. Molecular Phylogenetics and Evolution 17: 133–144. Google Scholar

54.

Tosi, A. J., Morales, J. C. and Melnick, D. J. 2002. Y-chromosome and mitochondrial markers in Macaca fascicularis indicate introgression with Indochinese M. mulatta and a biogeographic barrier in the Isthmus of Kra. International Journal of Primatology 23: 161–178. Google Scholar

Appendices

Supplementary Table S1.

Information on the samples used in mtDNA examination

st_ms2020-0078_001.gif

Supplementary Table S2.

List of mtDNA 223 haplotypes classified by multiple alignments of 409 D-loop reference data deposited in the GenBank database

st_ms2020-0078_002a.gif

Continued

st_ms2020-0078_002b.gif

Continued

st_ms2020-0078_002c.gif

Continued

st_ms2020-0078_002d.gif

Supplementary Table S3.

Results of hybrid judgement for males that carried the U type of Y chromosome in Table 4

st_ms2020-0078_003.gif
© The Mammal Society of Japan
Yoshi Kawamoto "Genetic Assessment on the Origin of Alien Macaques in the Boso Peninsula in Japan," Mammal Study 46(2), 173-186, (16 April 2021). https://doi.org/10.3106/ms2020-0078
Received: 4 August 2020; Accepted: 28 December 2020; Published: 16 April 2021
JOURNAL ARTICLE
14 PAGES


SHARE
ARTICLE IMPACT
Back to Top