600 lichens and 82 lichenicolous and allied fungi have been recorded from Eagle Hill in Steuben, ME, and its vicinity over the past 25 years, mainly as a consequence of courses and research centered upon the Eagle Hill Institute (formerly the Humboldt Field Research Institute). Of the 682 taxa listed, 331 have been recorded within the Institute's boundary, of which 27 were not found elsewhere in the vicinity; a further 66 taxa recorded but lacking voucher material are listed. One lichen, Lambiella fuscosora, and 7 lichenicolous fungi, Cornutispora pyramidalis, Epicladonia stenospora, Monodictys epilepraria, Muellerella polyspora, Taeniolella cladinicola, and Tremella coppinsii, are additional to the North American checklist; Lambiella fuscosora and Cornutispora pyramidalis are also recorded as new for Canada. Five lichens, Alyxoria ochrocheila, Cladonia albonigra, Ephebe solida, Myriolecis schofieldii, and Parmotrema stuppeum, are new for Maine. Notes on new records and interesting taxa are provided.