Since 2004, my students and I have caught and examined 1441 individual bats representing 7 species from mist-net surveys in Grant County, WI. Across all years and sites, Myotis lucifugus (Little Brown Bat) was the most frequently captured bat (59.6% of all captures). We captured over 78% (n = 1106) of bats within the first 120 min after sunset. Based on the capture of lactating females, the following species raise young in southwest Wisconsin: Eptesicus fuscus (Big Brown Bat), Perimyotis subflavus (Tri-colored Bat), Little Brown Bat, M. septentrionalis (Northern Long-eared Bat), Lasiurus borealis (Eastern Red Bat), and L. cinereus (Hoary Bat). Secondary sexual dimorphism was most evident in Big Brown Bats and Eastern Red Bats. Trends at 1 primary research site for the 3 most commonly captured species showed that, from 2007 to 2017, there was no significant change in number of bats captured per mist-net-meter-hour for Big Brown Bats and Eastern Red Bats. In contrast, and coincident with the occurrence of white-nose syndrome in Wisconsin, there was a signicant decline in the number of Little Brown Bats captured per mist-net-meter-hour over that same time period. Monitoring of bat populations in southwest Wisconsin should continue.