This study describes the fauna of odonates of nine localities in the Zapata peninsula in the South-west of Cuba, sampled in June 2017, January 2018, and September 2019. We failed to find Enallagma truncatum, an endemic species of the area, even though we visited the type locality, Zarabanda, in June and January. We found 37 species in 25 genera and five families, including the first records of 11 taxa for the area: Lestes forficula, L. tenuatus, Leptobasis candelaria, Neoneura maria, Aphylla caraiba, Celithemis eponina, Crocothemis servilia, Erythemis plebeja, Erythrodiplax berenice naeva, Perithemis domitia and Tramea onusta. A dichotomous key for the separation of the four species of females of Cuban Lestes is given.