Well preserved radiolarians of middle Permian age were extracted from a manganese carbonate nodule from the Nishiki Group of the Akiyoshi Belt in southwest Japan. These radiolarians consist of a middle Permian assemblage, including Parafollicucullus yanaharensis, P. lanceolatus, and Albaillella foremanae as well as two species newly described in this paper, P. babelis sp. nov. and P. grandis sp. nov. Because of their excellent state of preservation, we were able to study the structures of several species of Parafollicucullus in detail. Some have been previously recognized, including multilayered shells, a boundary structure (“pre-pseudoabdominal junction”) between the pre-pseudoabdomen and pseudoabdomen, a hole structure at the base of the wing, a “lingula” structure at the foot, and a “pleat” or folded structure resembling a skirt on the wing. These features should provide new criteria to distinguish species of Parafollicucullus.