Acetolysis has been widely used for the preparation of modern pollen samples since its introduction by Erdtman. It has several disadvantages in both safety and resultant changes to the size and, in some cases, structure of pollen grains. The enzymatic technique introduced by Schols and colleagues is tested on a fresh pollen sample, a honey sample and a bee pollen capsule. The technique increases processing time, but is completely non-toxic, easy to complete and comparable in cost to acetolysis. Even very fragile grains are not damaged by this technique.
How to translate text using browser tools
1 May 2017
An alternative to acetolysis: application of an enzyme-based method for the palynological preparation of fresh pollen, honey samples and bee capsules
Jennifer M.K. O'Keefe,
Carol L. Wymer
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
Palynology
Vol. 41 • No. 1–2
Feb-May 2017
Vol. 41 • No. 1–2
Feb-May 2017
cellulase
enzyme
melissopalynology
pectinase
pollen