How to translate text using browser tools
1 May 2000 Modulation of c-jun and c-fos Transcription by UVB and UVA Radiations in Human Dermal Fibroblasts and KB Cells
Marco Soriani, Vidya Hejmadi, Rex M. Tyrrell
Author Affiliations +

We have previously demonstrated that the oxidizing component of ultraviolet-A (UVA) plays a central role in the activation of the nuclear oncogene and transcription factor, c-fos, in cultured human skin fibroblasts. We have now shown that expression of both c-jun and c-fos (AP-1) family of transcription factors is modulated by short and long wavelength solar ultraviolet (UV) radiation in human fibroblasts and human KB cells. UVA radiation activated c-jun and c-fos in both fibroblasts and KB cells, whereas ultraviolet-B (UVB) radiation activates such oncogenes only in KB cells. Moreover, decreasing the intracellular levels of reducing equivalents in human fibroblasts by glutathione (GSH) depletion lowered the UVA dose threshold for c-jun and c-fos activation several-fold and greatly amplified the UVA-mediated activation of such genes. A more modest effect was observed in GSH-depleted KB cells. In both GSH-depleted fibroblasts and KB cells, UVB radiation failed to amplify c-jun and c-fos activation indicating that the oxidative component of UVB plays a minor role in the modulation of such oncogene expression. These findings clearly indicate that both c-jun and c-fos are activated by the oxidizing component of UVA radiation in human fibroblasts and KB cells, while UVB-mediated modulation seems to be restricted to human epithelial cells and does not involve oxidizing intermediates.

Marco Soriani, Vidya Hejmadi, and Rex M. Tyrrell "Modulation of c-jun and c-fos Transcription by UVB and UVA Radiations in Human Dermal Fibroblasts and KB Cells," Photochemistry and Photobiology 71(5), 551-558, (1 May 2000).<0551:MOCJAC>2.0.CO;2
Received: 6 August 1999; Accepted: 1 February 2000; Published: 1 May 2000

This article is only available to subscribers.
It is not available for individual sale.

Get copyright permission
Back to Top