1 September 2002 Apoptotic Response to Photodynamic Therapy versus the Bcl-2 Antagonist HA14-1
David Kessel, John J. Reiners
Author Affiliations +

In this study, murine leukemia L1210 cells were used to compare the effects of photodynamic therapy (PDT) with those of the apoptotic nonpeptidic Bcl-2 ligand ethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (HA14-1). The photosensitizing agent capronyloxy-tetrakis methyloxyethyl porphycene (CPO) was selected from a group of sensitizers previously shown to target the antiapoptotic protein Bcl-2 for photodamage. Like PDT with CPO, HA14-1 caused the rapid activation of procaspase-3, followed by the appearance of an apoptotic morphology within 60 min. Caspase activation after a sublethal dose of either PDT or HA14-1 was enhanced by staurosporine or the bile acid ursodeoxycholic acid. Moreover, PDT promoted procaspase activation and lethality of HA14-1 and vice versa. Effects of PDT versus HA14-1 could not be distinguished on the basis of the reactive oxygen species formation. Both caused the rapid oxidation of 2′,7′-dichlorofluorescein. These results are consistent with the hypothesis that Bcl-2 photodamage is a target for some photosensitizing agents.

David Kessel and John J. Reiners "Apoptotic Response to Photodynamic Therapy versus the Bcl-2 Antagonist HA14-1," Photochemistry and Photobiology 76(3), 314-319, (1 September 2002). https://doi.org/10.1562/0031-8655(2002)076<0314:ARTPTV>2.0.CO;2
Received: 25 March 2002; Accepted: 1 May 2002; Published: 1 September 2002

This article is only available to subscribers.
It is not available for individual sale.

Get copyright permission
Back to Top