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1 September 2002 Impairment of Eye Lens Cell Physiology and Optics by Broadband Ultraviolet A–Ultraviolet B Radiation
O. M. Oriowo, A. P. Cullen, J. G. Sivak
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The phototoxicity of ultraviolet A (UVA) alone and UVA plus ultraviolet B (UVB) combined on cultured porcine lenses was investigated by analyzing cellular function as measured with a fluorescence bioassay approach and optical integrity, in terms of sharpness of the lens focus as measured with a scanning laser system. The bioassay consisted of carboxyfluorescein diacetate-acetoxymethyl ester and alamarBlue fluorescent dyes. Aseptically dissected porcine lenses were maintained in modified medium 199 without phenol red supplemented with 1% penicillin–streptomycin and 4% porcine serum. At 1 week of preincubation, baseline measurements were obtained. Then the lenses were treated with single exposures of different UVA and UVB energy levels. The lenses treated with 86 J/cm2 UVA alone showed a significant (P < 0.05) decrease in cellular and optical integrity at 48 h after exposure, whereas those treated with 43 J/cm2 UVA alone did not show significant phototoxic effect. Lenses treated with 15.63 J/cm2 UVA plus 0.019 J/cm2 UVB combined showed significant adverse effects beginning from 48 h after exposure. Also, there was no recovery. These findings show that a high UVA dose alone and relatively low UVA in combination with low UVB radiant exposure can impair lens cellular and optical functions, respectively.

O. M. Oriowo, A. P. Cullen, and J. G. Sivak "Impairment of Eye Lens Cell Physiology and Optics by Broadband Ultraviolet A–Ultraviolet B Radiation," Photochemistry and Photobiology 76(3), 361-367, (1 September 2002).<0361:IOELCP>2.0.CO;2
Received: 14 March 2002; Accepted: 1 June 2002; Published: 1 September 2002

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